Capillary electrophoretic determination of resveratrol in wines.
Journal of agricultural and food chemistry
confidence
Key findings
Capillary electrophoretic method developed to quantify trans- and cis-resveratrol in wines; analytical assay only, no biological endpoints.
View source on PubMed (PMID 10552635) ↗
- Sample size
- 26 wines
- Population
- N/A (analytical method paper, 26 wine samples)
- Dosing
- N/A
- Duration
- N/A
- Route
- N/A
- Blinding
- not_reported
- Controls
- none
- Drug class
- polyphenol
Full abstract
A rapid and sensitive capillary electrophoretic method for analysis of resveratrol in wine was established. The protocol consists of sample preparation using a C-18 solid-phase extraction cartridge. Baseline separation of trans- and cis-resveratrol from other compounds in wine was achieved in approximately 8 min using a micellar mode. The limits of detection for trans- and cis-resveratrol were 0.1 and 0.15 micromol/L, respectively. Recovery rates for trans-resveratrol using the protocol described ranged from 94.6 +/- 8.5 to 101.9 +/- 7.2%. These procedures were used to analyze the trans- and cis-resveratrol levels in 26 wines. It was found that the concentration of trans-resveratrol ranged from 0.987 to 25.4 micromol/L, whereas the concentration of cis-resveratrol was much lower.