Simultaneous determination of cis- and trans-resveratrol in wines by capillary zone electrophoresis.
The Analyst
confidence
Key findings
Capillary zone electrophoresis method for quantifying cis- and trans-resveratrol in wines; analytical/methods paper with no clinical endpoints.
View source on PubMed (PMID 10563045) ↗
- Sample size
- N/A
- Population
- N/A (analytical method paper, red wine samples)
- Dosing
- N/A
- Duration
- N/A
- Route
- N/A
- Blinding
- not_reported
- Controls
- none
- Drug class
- polyphenol
Full abstract
A capillary zone electrophoresis method for determining cis- and trans-resveratrol isomers is proposed. Optimal conditions for the quantitative separation were investigated. A background electrolyte solution consisting of 40 mM borate buffer adjusted to pH 9.5, hydrodynamic injection and 5 kV of separation voltage were used. Good linearity and precision were obtained for the two isomers. Detection limits of 0.06 mg L-1 for trans-resveratrol and 0.08 mg L-1 for cis-reveratrol were obtained. The developed method is rapid and sensitive and it has been applied to determine cis- and trans-resveratrol in several red wines. The samples were purified and enriched by passing them through a preconditioned C18 cartridge and eluting the isomers with acetonitrile-water (3 + 7).