Spontaneous galactosylation of agalactoglycoproteins in colostrum.
FEBS letters
confidence
Key findings
Spontaneous galactosylation of GlcNAc residues occurs in bovine colostrum via endogenous UDP-Gal (200-600 microM); biochemical/methods study with no clinical endpoints.
View source on PubMed (PMID 10812067) ↗
- Sample size
- Not reported
- Population
- Bovine colostrum samples (in vitro biochemical study)
- Dosing
- Not applicable
- Duration
- Not reported
- Route
- Not applicable
- Blinding
- not_reported
- Controls
- none
- Drug class
- biological supplement
Full abstract
We have found that spontaneous galactosylation of GlcNAc residues occurs in bovine colostrum, but not in dialyzed colostrum, without adding UDP-Gal as a donor substrate. UDP-Gal was shown to be present in bovine colostrum at a level ranging from 200 to 600 microM. When a tracer UDP-[(14)C]Gal was added to the dialyzed colostrum together with a Gal beta1,4-specific beta-galactosidase, remarkable incorporation of radioactivity into 24-28 kDa and 33 kDa RCA1-positive glycoproteins was demonstrated by SDS-PAGE/autoradiography. Some 100-140 kDa agalactoglycoproteins of a CHO mutant cell line were also galactosylated on a blotted membrane by the incubation in the colostrum.