Gonadorelinobservational1975
A specific radio-immunoassay for gonadotrophin-releasing hormone.
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde
confidence
Key findings
Developed specific radio-immunoassay for GnRH sensitive to 5 pg/tube; measurable in hypothalamic extracts but undetectable in plasma/urine.
View source on PubMed (PMID 1101389) ↗
- Sample size
- Not reported
- Population
- Normal subjects and menopausal women (assay development study)
- Dosing
- Not applicable
- Duration
- Not reported
- Route
- Not applicable
- Blinding
- not_reported
- Controls
- none
- Drug class
- GnRH analog
Full abstract
A specific antiserum has been made to synthetic gonadotrophin-releasing hormone (GnRH) conjugated to keyhole limpet haemocyanin and appears to be directed against amino acids 6-8 of this decapeptide. This has allowed the development of a radio-immunoassay for GnRH sensitive to 5 picograms per tube. Although it is easily measurable in hypothalamic extracts, we have failed to detect GnRH in plasma and urine from normal subjects and menopausal women.