Separation of lactoferrin-a and -b from bovine colostrum.
Journal of dairy science
confidence
Key findings
Lactoferrin separated into lactoferrin-a (MW 84,000) and lactoferrin-b (MW 80,000) by ion-exchange chromatography; methods/analytical study.
View source on PubMed (PMID 11049060) ↗
- Sample size
- 6 cows
- Population
- Bovine colostrum and milk samples from cows
- Dosing
- N/A
- Duration
- Normal lactational period
- Route
- N/A
- Blinding
- not_reported
- Controls
- none
- Drug class
- biological supplement
Full abstract
Bovine lactoferrin was separated into lactoferrin-a and lactoferrin-b from bovine colostrum. Lactoferrin-a was eluted at 0.38 M NaCl and lactoferrin-b was eluted at 0.43 M NaCl by carboxymethyl cation-exchange chromatography at pH 7.7, 0.05 M phosphate buffer. The molecular weights were estimated at 84,000 for lactoferrin-a and 80,000 for lactoferrin-b. Lactoferrin-a contents were 258.0 mg/L and lactoferrin-b contents were 524.3 mg/L of colostrum for cow 19. From colostrum to normal milk, total lactoferrin was from 17.1 to 129.4 mg/L during the normal lactational period; however, lactoferrin did not separate clearly into lactoferrin-a and lactoferrin-b. The lactoferrin-a measured from six cows was 258.0, 114.0, 112.8, 64.0, 59.7, and 22.4 mg/ L and the lactoferrin-b 524.3, 331.8, 184.7, 170.7, 129.3, and 44.0 mg/L, respectively. The average was 105.2 mg (31.3%) for lactoferrin-a and 230.8 mg (68.7%) for lactoferrin-b.