Liquid chromatography-mass spectrometry detection of corticotropin-releasing hormone and proopiomelanocortin-derived peptides in human skin.
The Journal of clinical endocrinology and metabolism
confidence
Key findings
Analytical LC-MS study identifying endogenous CRH, ACTH 1-39, ACTH 1-13, and alpha-MSH peptides in human skin; no clinical/biological endpoints.
View source on PubMed (PMID 11061505) ↗
- Sample size
- Not reported
- Population
- Human skin tissue extracts
- Dosing
- Not applicable
- Duration
- Not applicable
- Route
- Not applicable
- Blinding
- not_reported
- Controls
- none
- Drug class
- anti-inflammatory peptide
Full abstract
We have previously shown expression of CRH and POMC genes and peptides in the human skin. To ascertain the identity of those peptides, we used methods of peptide extraction and purification combined with the highly specific technique of liquid chromatography-mass spectrometry. Testing extracts of human skin, we identified endogenous peptides with masses and retention times corresponding to CRH, ACTH 1-39, ACTH 1-13, and alpha-MSH standards. Thus, conclusive evidence is provided for the presence of CRH and the POMC-derived ACTH 1-39, ACTH 1-13, and alpha-MSH peptides in human skin. Direct identification of these peptides is consistent with translation of the corresponding genes, and it also suggests intermediate pituitary lobe-like POMC peptide processing.