Effect of six flavonoids on proliferation of hepatic stellate cells in vitro.
Acta pharmacologica Sinica
confidence
Key findings
Quercetin and five other flavonoids inhibited HSC proliferation concentration-dependently; quercetin IC50 18.52 (MCM) and 9.48 µmol/L (PDGF).
View source on PubMed (PMID 11324426) ↗
- Sample size
- Not reported (in vitro cell culture)
- Population
- Hepatic stellate cells (HSC-T6 cell line) in vitro
- Dosing
- 6.25-50 µmol/L
- Duration
- Not reported
- Route
- in vitro (cell culture medium)
- Blinding
- not_reported
- Controls
- none
- Drug class
- flavonoid
Measured endpoints
- Proliferation of HSC-T6 cells stimulated by serumDecreasedhepaticnot_reportedeffect: concentration-dependent inhibition (6.25-50 µmol/L)
- Proliferation of HSC-T6 cells stimulated by macrophage conditioned medium (MCM)Decreasedhepaticnot_reportedeffect: IC50 18.52 µmol/L (quercetin)
- Proliferation of HSC-T6 cells stimulated by platelet-derived growth factor (PDGF)Decreasedhepaticnot_reportedeffect: IC50 9.48 µmol/L (quercetin)
Full abstract
To study the effects of six flavonoids (fisetin, quercetin, apigenin, phloretin, hesperetin, and chalcone) on proliferation of hepatic stellate cell (HSC-T6 cells). Cell proliferation was measured by crystal violet staining assay. Fisetin, quercetin, apigenin, phloretin, hesperetin, chalcone (6.25-50 mumol.L-1) inhibited the proliferation of HSC-T6 cells stimulated by serum, macrophage conditioned medium (MCM) and platelet-derived growth factor (PDGF) in a concentration-dependent manner. In the MCM-stimulated proliferation experiment, their IC50 were 21.48, 18.52, 19.75, 22.32, 30.32, and 30.85 mumol.L-1, respectively. In the PDGF-stimulated proliferation experiment, their IC50 were 9.47, 9.48, 9.25, 12.25, 25.22, and 30.40 mumol.L-1, respectively. The six flavonoids inhibited the proliferation of hepatic stellate cells.