Cloning, sequencing, and expression of human gonadotropin releasing hormone (GnRH) receptor.
Biochemical and biophysical research communications
confidence
Key findings
Cloned and characterized human GnRH receptor cDNA; 7-transmembrane G protein-coupled receptor expressed in pituitary, ovary, testis, breast, prostate.
View source on PubMed (PMID 1333190) ↗
- Sample size
- N/A
- Population
- N/A (molecular cloning study, human GnRH receptor cDNA)
- Dosing
- N/A
- Duration
- N/A
- Route
- N/A
- Blinding
- not_reported
- Controls
- none
- Drug class
- GnRH analog
Full abstract
Gonadotropin releasing hormone is a hypothalamic decapeptide that stimulates the release of gonadotropic hormones from the anterior pituitary gland. Therapeutically, the human pituitary GnRH receptor is the target of agonists used in the suppression of prostate cancer. Here we report the isolation of a cDNA representing this receptor. It encodes a protein with a transmembrane topology similar with that of other G protein-coupled, 7-transmembrane receptors. Binding studies of the cloned receptor demonstrate high affinity and pharmacological properties similar with the native human pituitary GnRH receptor. Northern blot and reverse transcriptase/PCR analysis revealed that its mRNA is expressed in pituitary, ovary, testis, breast, and prostate but not in liver and spleen. Availability of a human GnRH receptor cDNA should permit the design of improved analogs for therapeutic applications.