Characterization of gonadotropin-releasing hormone binding to pituitary receptors in the gilthead seabream (Sparus aurata).
Biology of reproduction
confidence
Key findings
Characterized single class of high-affinity GnRH receptors in seabream pituitary (Ka=0.567x10^9 M-1, Bmax=1091 fmol/mg); methods/assay paper.
View source on PubMed (PMID 1337273) ↗
- Sample size
- Not reported (in vitro receptor binding)
- Population
- Gilthead seabream (Sparus aurata) pituitary tissue
- Dosing
- Ligand concentrations up to 4 x 10^-9 M
- Duration
- 1 h equilibrium binding at 4°C
- Route
- In vitro radioligand binding assay
- Blinding
- not_reported
- Controls
- none
- Drug class
- GnRH analog
Full abstract
Receptors for GnRH in the pituitary of Sparus aurata were characterized using iodinated [D-Ala6-Pro9-NEt]-LHRH (GnRHa). Equilibrium binding of the ligand to the receptor was achieved after 1 h at 4 degrees C. Binding of the radioligand was a function of tissue concentration, with a linear correlation over the range of one-sixteenth to three-fourths pituitary per tube. Displacement experiments with salmon GnRH (sGnRH), GnRHa, as well as unrelated peptides demonstrated the specificity of the receptors. Binding was found to be saturable at ligand concentrations of 4 x 10(-9) M. Scatchard analysis of the saturation data suggested the presence of a single class of high-affinity sites (Ka = 0.567 +/- 0.136 x 10(9) M-1, Bmax = 1091 +/- 207 fmol/mg protein).