NAD+observational2010
Effect of NAD on Malate Oxidation in Intact Plant Mitochondria.
Plant physiology
confidence
Key findings
NAD(+) addition increased malate oxidation in potato tuber mitochondria but not mung bean; traced to low endogenous NAD(+) in potato mitochondria.
View source on PubMed (PMID 16661409) ↗
- Sample size
- Not reported
- Population
- Plant mitochondria (potato tuber and mung bean hypocotyl)
- Dosing
- Added NAD(+) to mitochondria
- Duration
- Not reported
- Route
- In vitro (mitochondrial incubation)
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
Potato tuber mitochondria oxidizing malate respond to NAD(+) addition with increased oxidation rates, whereas mung bean hypocotyl mitochondria do not. This is traced to a low endogenous content of NAD(+) in potato mitochondria, which prove to take up added NAD(+). This mechanism concentrates NAD(+) in the matrix space. Analyses for oxaloacetate and pyruvate (with pyruvate dehydrogenase blocked) are consistent with regulation of malate oxidation by the internal NAD(+)/NADH ratio.