Conformational flexibility of luteinizing hormone-releasing hormone in aqueous solution. A carbon-13 spin-lattice relaxation time study.
Biochemistry
confidence
Key findings
13C NMR and T1 relaxation study showed LH-RH is a flexible molecule in solution with segmental motion along backbone and nonaromatic side chains.
View source on PubMed (PMID 170960) ↗
- Sample size
- Not reported
- Population
- In vitro aqueous solution study of LH-RH and homologous peptides
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- GnRH analog
Full abstract
The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the T1 data the rates of overall molecular motion and intramolecular motion of side chains have been estimated. LH-RH is a flexible molecule in solution, having segmental motion along the backbone as well as in the nonaromatic side chains.