Hydrolytic cleavage of DNA by quercetin manganese(II) complexes.
Colloids and surfaces. B, Biointerfaces
confidence
Key findings
Quercetin manganese(II) complexes promote DNA hydrolytic cleavage (single and double strand breaks); no clinical/biological endpoints reported.
View source on PubMed (PMID 17234390) ↗
- Population
- In vitro (plasmid DNA)
- Dosing
- 100 microM complexes
- Route
- In vitro incubation
- Blinding
- not_reported
- Controls
- not_reported
- Drug class
- flavonoid
Full abstract
Quercetin manganese(II) complexes were investigated focusing on its DNA hydrolytic activity. The complexes successfully promote the cleavage of plasmid DNA, producing single and double DNA strand breaks. The amount of conversion of supercoiled form (SC) of plasmid DNA to the nicked circular form (NC) depends on the concentration of the complex as well as the duration of incubation of the complexes with DNA. The maximum rate of conversion of the supercoiled form to the nicked circular form at pH 7.2 in the presence of 100 microM of the complexes is found to be 1.32 x 10(-4) s(-1). The hydrolytic cleavage of DNA by the complexes was supported by the evidence from free radical quenching, thiobarbituric acid-reactive substances (TBARS) assay and T4 ligase ligation.