Regeneration of NAD(P)H by immobilized whole cells of Clostridium butyricum under hydrogen high pressure.
Biotechnology and bioengineering
confidence
Key findings
Immobilized Cl. butyricum reduced NAD+/NADP+ to NADH under 100 atm H2; 89% activity retained after 4 cycles; produced L-alanine from H2, ammonium, pyruvate.
View source on PubMed (PMID 18553815) ↗
- Sample size
- Not reported
- Population
- Immobilized whole cells of Clostridium butyricum (in vitro)
- Dosing
- NAD(+) 6.4 mumole; hydrogen pressure 100 atm
- Duration
- 5 h reactions repeated 4 times
- Route
- In vitro biocatalysis
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
Immobilized whole cells of Clostridium butyricum reduced both NAD(+) and NADP(+) in the presence of hydrogen at a pressure of 100 atm. The NAD(+) and NADP(+) reduction activities were 4.45 and 4.30 U/g dry cells, respectively [U = NAD(P)H regenerated, mu mol/min]. The amount of NADH regenerated by immobilized cells increased with increasing hydrogen pressure above 10 atm. Immobilized cells (6 mg dry cells) of Cl. butyricum completely converted NAD(+) (6.4 mumole) to NADH for 5 h, whereas only 60% of NAD(+) were reduced by free cells. Immobilized cells retained 89% activity after the 5-h reactions were repeated 4 times. L-Alanine was continuously produced at the rate of 12.8 mumol/min g dry cells from hydrogen, ammonium, and pyruvate with immobilized Cl. butyricum-alanine dehydrogenase.