NAD+observational1978
NAD nucleosidase of Agkistrodon bilineatus venom.
The American journal of tropical medicine and hygiene
confidence
Key findings
NAD nucleosidase activity identified in Agkistrodon bilineatus venom; activity thermolabile; no clinical or biological endpoints reported.
View source on PubMed (PMID 206161) ↗
- Population
- Agkistrodon bilineatus venom (in vitro enzyme assay)
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) activity in Agkistrodon bilineatus venom was observed. Using the cyanide assay method at pH 7.9, lyophilized crude venom had an activity of 0.19 units/mg. Chromatography of the crude venom on DEAE Sephadex A-50 with ammonium acetate buffer by two stage elution yielded 13 fractions. Peak NAD nucleosidase activity occurred at Fraction X. Crude venom and Fraction X NAD nucleosidase activities were thermolabile.