A glycine residue essential for high ivermectin sensitivity in Cys-loop ion channel receptors.
International journal for parasitology
confidence
Key findings
A glycine residue at a specific transmembrane domain location is essential for high ivermectin sensitivity in Cys-loop receptors; no clinical/biological endpoints reported.
View source on PubMed (PMID 20713056) ↗
- Sample size
- Not reported
- Population
- Nematode Cys-loop ion channel receptors (in vitro)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- antiparasitic
Full abstract
Ivermectin exerts its anthelmintic effect by activating nematode Cys-loop glutamate-gated receptors. Here we show that a glycine residue at a specific transmembrane domain location is essential for high ivermectin sensitivity in both glycine- and glutamate-gated Cys-loop receptors. We also show that ivermectin sensitivity can be conferred on an ivermectin-insensitive receptor by introducing a glycine at this position. Furthermore, comparison of amino acid sequences of ivermectin-sensitive and -resistant receptors reveals that the presence of a glycine reliably predicts ivermectin sensitivity. By providing a means of identifying ivermectin-sensitive receptors, this finding should help in characterising ivermectin-resistance mechanisms and identifying new anthelmintic targets.