Frex and FrexH: Indicators of metabolic states in living cells.
Bioengineered bugs
confidence
Key findings
Review of genetically encoded fluorescent NADH sensors for real-time, quantitative measurement of NADH in subcellular compartments; no clinical/biological endpoints reported.
View source on PubMed (PMID 22572785) ↗
- Sample size
- Not reported
- Population
- Living cells (in vitro)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
Reduced nicotinamide adenine dinucleotide (NADH) and its oxidized form play central roles in energy and redox metabolisms. For many years, researchers have relied on the weak NADH endogenous fluorescence signal to determine the NADH level in living cells. We recently reported a series of genetically encoded fluorescent sensors highly specific for NADH. These sensors allow real-time, quantitative measurement of this significant molecule in different subcellular compartments. In this study, we provide a more detailed discussion of the benefits and limitations of these genetically encoded fluorescent sensors. These sensors are utilized in most laboratories without the need for sophisticated instruments because of their superior sensitivity and specificity. They are also viable alternatives to existing techniques for measuring the endogenous fluorescence of intracellular NAD(P)H.