Creatine ethyl ester: a new substrate for creatine kinase.
Molekuliarnaia biologiia
confidence
Key findings
Purified creatine kinase can phosphorylate creatine ethyl ester; K(m), V(max), temperature and pH optima determined. No clinical/biological endpoints reported.
View source on PubMed (PMID 22642114) ↗
- Population
- In vitro (purified creatine kinase enzyme)
- Blinding
- not_reported
- Controls
- none
- Drug class
- nootropic
Full abstract
The creatine kinase/phosphocreatine system plays a key role in cell energy buffering and transport, particularly in cells with high or fluctuating energy requirements, like neurons, i.e. it participates in the energetic metabolism of the brain. Creatine depletion causes several nervous system diseases, alleviated by phosphagen supplementation. Often, the supplementation contains both creatine and creatine ethyl ester, known to improve the effect of creatine through an unknown mechanism. In this work we showed that purified creatine kinase is able to phosphorilate the creatine ethyl ester. The K(m) and V(max) values, as well as temperature and pH optima were determined. Conversion of the creatine ethyl ester into its phosphorylated derivative, sheds light on the role of the creatine ethyl ester as an energy source in supplementation for selected individuals.