Nanoparticle-tethered NAD(+) with in situ cofactor regeneration.
Biotechnology letters
confidence
Key findings
Nanoparticle-tethered NAD+ enabled efficient cofactor regeneration and produced 0.16 M L-lactate; retained 60% activity after six reuses.
View source on PubMed (PMID 23417259) ↗
- Sample size
- N/A
- Population
- In vitro biochemical reaction system
- Dosing
- 0.2 M pyruvate, 3 M formate
- Duration
- Six reuses
- Route
- Immobilized on silica nanoparticles
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
A new and simple route for the preparation of immobilized NAD(+) on carboxyl-activated silica nanoparticles activated by γ-aminpropyltriethoxysilane and glutaric anhydride was developed. In addition, formate dehydrogenase, keto-reductase and the silica nanoparticle-attached NAD(+) were applied to catalyze the coupled reactions for production of L-lactate with the cofactor regenerated within the reaction cycle. As indicated by thermogravimetric analysis and FT-IR, the silica nanoparticles were successfully activated and the loading of carboxyl groups was 0.53 mmol g(-1) particle. The amount of immobilized NAD(+) on the support was 73 mg g(-1) particle. With 0.2 M pyruvate and 3 M formate, 0.16 M L-lactate was produced after the coupled reactions. The immobilized system showed excellent efficiency and stabilities in recycling, and it retained 60 % residual activity even after six reuses.