Accurate measurement of nicotinamide adenine dinucleotide (NAD⁺) with high-performance liquid chromatography.
Methods in molecular biology (Clifton, N.J.)
confidence
Key findings
Describes a highly quantitative HPLC method for measuring NAD+ levels in cells and tissues; no clinical/biological endpoints reported.
View source on PubMed (PMID 24014409) ↗
- Sample size
- Not reported
- Population
- Cells and tissues (in vitro and in vivo)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- not_reported
- Drug class
- peptide
Full abstract
Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD(+) levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD(+) measurement, which enables us to analyze various pathophysiological changes in NAD(+) levels in vitro and in vivo.