Orientation of the porphyrin ring in artificial chlorophyll membranes.
The Journal of membrane biology
confidence
Key findings
Photometric method measures dichroism in Soret and red bands of chlorophylla in lipid bilayers; calculates porphyrin ring angle with membrane surface (~44-49°).
View source on PubMed (PMID 24177647) ↗
- Sample size
- Not reported
- Population
- Artificial chlorophyll lipid bilayer membranes (in vitro)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- not_reported
- Drug class
- porphyrin pigment
Full abstract
A sensitive photometric method is described by which the dichroism of lipid bilayer membranes in aqueous phase can be measured. The method is applied to black films with incorporated chlorophylla andb. With chlorophylla a relatively large dichroism is found in the Soret band and a much weaker dichroism in the red band. From the experimental data, the angles β B and β R between the "blue" and "red" transition moments and the membrane can be obtained. β B and β R are then used to calculate the angle γ of the porphyrin ring with respect to the membrane surface. For chlorophylla and three different lipids, values of γ between 44 and 49° are found.