NAD+observational2014
An improved fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran: its origin and application.
Chemical communications (Cambridge, England)
confidence
Key findings
Developed fluorogenic NAD(P)+ detection method using 2-acetylbenzofuran; analytical method paper, no clinical/biological endpoints.
View source on PubMed (PMID 24177803) ↗
- Sample size
- Not reported
- Population
- In vitro (microtiter-plate format)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
We have developed a fluorogenic NAD(P)(+) detection method using 2-acetylbenzofuran. The reaction of NAD(P)(+) with 2-acetylbenzofuran produced a fluorescent product, allowing the highly-sensitive and quick detection of NAD(P)(+). This method was successfully applied to the detection of P450 substrates in the microtiter-plate format.