Optimization of enzyme-assisted extraction and characterization of polysaccharides from Hericium erinaceus.
Carbohydrate polymers
confidence
Key findings
Optimized EAE conditions for H. erinaceus polysaccharides extraction (pH 5.71, 52.03°C, 33.79 min) yielded 13.46% HEP, 67.72% higher than HWE; analytical characterization only.
View source on PubMed (PMID 24299817) ↗
- Sample size
- Not reported
- Population
- Not applicable (analytical study of Hericium erinaceus polysaccharides)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- adaptogen
Full abstract
The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03°C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.