Polyadenylated mRNA for the light-harvesting chlorophyll a/b protein : Its presence in green and absence in chloroplast-free plant cells.
Planta
confidence
Key findings
Poly(A)RNA from green plants directs synthesis of LHCP precursor (29,000 MW), absent in chloroplast-free Petroselinum poly(A)RNA.
View source on PubMed (PMID 24310185) ↗
- Sample size
- Not reported
- Population
- Green plants (parsley, pea, barley) and chloroplast-free Petroselinum cell culture
- Dosing
- Not reported
- Duration
- Not reported
- Route
- In vitro translation (wheat germ cell-free system)
- Blinding
- not_reported
- Controls
- none
- Drug class
- porphyrin pigment
Full abstract
In thylakoid membranes isolated from green plants of parsley, pea, and barley, the light-harvesting chlorophyll a/b protein complex (LHCP, mol. weight: 25,000), is a major constituent. Poly(A)RNA isolated from these species was translated in a wheat germ, cell-free system. The in vitro translation products were treated with antibodies raised against the LHCP. This treatment resulted in the precipitation of a precursor protein (mol. weight: 29,000). Poly(A)RNA was also prepared from a cell culture ofPetroselinum that does not develop chloroplasts upon illumination. This poly(A)RNA is capable of stimulating amino acid incorporation in the in vitro translation system, however, it does not direct the synthesis of LHCP.