Delta-sleep inducing peptide entrapment in the charged macroporous matrices.
Materials science & engineering. C, Materials for biological applications
confidence
Key findings
DSIP nearly 100% entrapped in positively charged Co-DMAEMA-MBAA matrix; release driven by ionic strength in saline. Methods/materials paper.
View source on PubMed (PMID 25063142) ↗
- Sample size
- N/A
- Population
- Not applicable (in vitro materials/chemistry study)
- Dosing
- N/A
- Duration
- N/A
- Route
- N/A
- Blinding
- not_reported
- Controls
- active_comparator
- Drug class
- sleep-modulating peptide
Full abstract
Various biomolecules, for example proteins, peptides etc., entrapped in polymer matrices, impact interactions between matrix and cells, including stimulation of cell adhesion and proliferation. Delta-sleep inducing peptide (DSIP) possesses numerous beneficial properties, including its abilities in burn treatment and neuronal protection. DSIP entrapment in two macroporous polymer matrices based on copolymer of dimethylaminoethyl methacrylate and methylen-bis-acrylamide (Co-DMAEMA-MBAA) and copolymer of acrylic acid and methylen-bis-acrylamide (Co-AA-MBAA) has been studied. Quite 100% of DSIP has been entrapped into positively charged Co-DMAEMA-MBAA matrix, while the quantity of DSIP adsorbed on negatively charged Co-AA-MBAA was only 2-6%. DSIP release from Co-DMAEMA-MBAA was observed in saline solutions (0.9% NaCl and PBS) while there was no DSIP release in water or 25% ethanol, thus ionic strength was a reason of this process.