Collagen Peptidesobservational1990
[Isolation and purification of biopolymers using an affinity chromatography method. X. A biospecific approach to preparing collagen-like peptides containing segments binding fibronectin].
Bioorganicheskaia khimiia
confidence
Key findings
Biospecific affinity chromatography method proposed to isolate collagen-like peptides containing fibronectin-binding site; biochemical isolation method.
View source on PubMed (PMID 2624589) ↗
- Sample size
- Not reported
- Population
- Not applicable (biochemical isolation method)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- supplement
Full abstract
A biospecific method for one-stage isolation of collagen peptides containing the fibronectin binding site is proposed. alpha 1CB7-peptide of the type I collagen cyanogen bromide cleavage was isolated by means of affinity chromatography on adsorbents containing an immobilized gelatin-binding domain (45 kDa) of fibronectin. The method allows one to obtain, in a short time, highly purified preparation of alpha 1CB7-peptide necessary for biochemical studies.