NAD+observational2016
Electrocatalytic interconversion of NADH and NAD(+) by Escherichia coli flavohemoglobin.
Chemical communications (Cambridge, England)
confidence
Key findings
E. coli flavohemoglobin electrocatalytically interconverts NADH and NAD+ at heme potentials; methods development for bioelectrodes.
View source on PubMed (PMID 26389555) ↗
- Population
- In vitro (E. coli flavohemoglobin at electrodes)
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
E. coli flavohemoglobin, oriented at electrodes via amphiphilic polymyxin B, electrocatalytically interconverts NADH and NAD(+) at its heme potentials operating as an electron transfer relay between the electrode and the protein FAD, where NADH/NAD(+) is transformed. The results are crucial for the development of NAD(+)-dependent bioelectrodes for biosynthesis, biosensors and biofuel cells.