Functional expression of rat pituitary gonadotrophin-releasing hormone receptors in Xenopus oocytes.
Journal of molecular endocrinology
confidence
Key findings
Functional GnRH receptor expression in Xenopus oocytes demonstrated by GnRH-induced membrane depolarization; no clinical/biological endpoints reported.
View source on PubMed (PMID 2908232) ↗
- Sample size
- not_reported
- Population
- Xenopus oocytes injected with rat anterior pituitary poly(A)+ mRNA
- Dosing
- GnRH 10nM - 1 microM; buserelin 1nM - 1 microM; GnRH antagonist 1 microM; TRH 10nM - 1 microM
- Duration
- not_reported
- Route
- in vitro mRNA injection
- Blinding
- not_reported
- Controls
- control oocytes without mRNA injection
- Drug class
- GnRH analog
Full abstract
Expression of receptors for the hypothalamic regulatory peptide, gonadotrophin-releasing hormone (GnRH), was investigated by intracellular recording from Xenopus oocytes injected with poly(A)+ mRNA isolated from rat anterior pituitary glands. Membrane depolarizations were induced in oocytes in a dose-dependent fashion following the application of GnRH (10nM - 1 microM) or a GnRH superactive agonist, buserelin (1nM - 1 microM). The response was reversibly blocked by the addition of a GnRH antagonist (1 microM). TRH (10nM - 1 microM) had no effect on most of these oocytes. In contrast, some other oocytes which showed no responses to GnRH or to the GnRH agonist, displayed depolarizing responses to TRH (10nM - 1 microM). A relatively small number of oocytes responded to both ligands. Control oocytes did not respond to the GnRH analogues or to TRH. This successful expression of the GnRH receptor could provide a new approach to the study of the receptor, and serve as a means for the isolation and cloning of the encoding genes.