A high-throughput method for screening of L-tyrosine high-yield strains by Saccharomyces cerevisiae.
The Journal of general and applied microbiology
confidence
Key findings
High-throughput biosensor screening method for L-tyrosine high-yield yeast strains using betaxanthin fluorescence; no clinical/biological endpoints.
View source on PubMed (PMID 29695662) ↗
- Sample size
- Not reported
- Population
- Saccharomyces cerevisiae mutant library (in vitro)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- amino acid
Full abstract
A biosensor screening assay based on the synthesis of betaxanthin was applied to relatively high throughput screening of the L-tyrosine mutant library. In the assays, fluorescence output showed a linear relationship between extracellular L-tyrosine content and yellow pigment formation. In addition, the yellow pigment accumulation of the L-tyrosine high-yield strain can be easily distinguished with the naked eye compared with the wild-type strain. As a result, numerous mutants that exhibited significantly increased coloration, were screened out after random mutagenesis, and p-coumaric acid production in mutants NK-A3 and NK-B4, were remarkably improved by 4-fold more than that of the wild-type strain. In general, this study provides a novel strategy for screening mutant libraries in the search for highly L-tyrosine-producing strains.