Solid phase extraction system for vitamin D and its major metabolites in human plasma.
Journal of chromatography
confidence
Key findings
New solid-phase extraction method for vitamin D and metabolites in plasma; no clinical/biological endpoints reported.
View source on PubMed (PMID 2999172) ↗
- Sample size
- Not reported
- Population
- Human plasma samples
- Dosing
- Not applicable
- Duration
- Not reported
- Route
- Not applicable
- Blinding
- not_reported
- Controls
- active_comparator
- Drug class
- fat-soluble vitamin
Full abstract
A new procedure using C18 and silica cartridges for the extraction and subsequent separation of vitamin D and its major metabolites from plasma has been developed and compared to a conventional extraction procedure with respect to lipophilic material extracted as evaluated by high-performance liquid chromatographic profiles. The C18 cartridges were efficient in extracting all compounds tested while subsequent chromatography of the extract on silica cartridges was effective in resolving vitamin D and its metabolites based on increasing polarity. High-performance liquid chromatographic profiles of each silica cartridge fraction clearly demonstrated that the newly conceived solid phase extraction was superior to conventional extraction methods with respect to cleanliness of sample fractions. This difference in lipophilic load between the new and conventional extraction systems was most apparent in the vitamin D and 25-hydroxyvitamin D containing fractions. The new extraction system can be used when total extraction and subsequent analysis of vitamin D and its major metabolites is desired.