Sensitivity to NN'-dicyclohexylcarbodi-imide of proton translocation by mitochondrial NADH:ubiquinone oxidoreductase.
The Biochemical journal
confidence
Key findings
DCCD caused 84% decrease in H+/e- ratio of NADH:cytochrome c reduction vs 49% for succinate:cytochrome c, indicating a DCCD-sensitive proton channel in NADH:ubiquinone oxidoreductase.
View source on PubMed (PMID 3026336) ↗
- Sample size
- Not reported
- Population
- Isolated rat liver mitochondria (in vitro)
- Dosing
- NN'-Dicyclohexylcarbodi-imide (DCCD) - dose not specified
- Duration
- Not reported
- Route
- In vitro
- Blinding
- not_reported
- Controls
- none
- Drug class
- mitochondrial cofactor
Full abstract
Proton extrusion during ferricyanide reduction by NADH-generating substrates or succinate was studied in isolated rat liver mitochondria with the use of optical indicators. NN'-Dicyclohexylcarbodi-imide (DCCD) caused a decrease of 84% in the H+/e- ratio of NADH:cytochrome c reduction, but a decrease of only 49% in that of succinate:cytochrome c reduction, even though electron transfer was decreased equally in both spans. The data indicate that a DCCD-sensitive channel operates in the NADH:ubiquinone oxidoreductase region of the respiratory chain.