Polymer-Based Module for NAD+ Regeneration with Visible Light.
Chembiochem : a European journal of chemical biology
confidence
Key findings
Polymer-based visible-light photocatalytic module regenerates NAD+ from NADH and oxidizes model substrate with glycerol dehydrogenase; no clinical/biological endpoints.
View source on PubMed (PMID 30883002) ↗
- Population
- In vitro synthetic/chemical system
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
The regeneration of enzymatic cofactors by cell-free synthetic modules is a key step towards producing a purely synthetic cell. Herein, we demonstrate the regeneration of the enzyme cofactor NAD+ by photo-oxidation of NADH under visible-light irradiation by using metal-free conjugated polymer nanoparticles. Encapsulation of the light-active nanoparticles in the lumen of polymeric vesicles produced a fully organic module able to regenerate NAD+ in an enzyme-free system. The polymer compartment conferred physical and chemical autonomy to the module, allowing the regeneration of NAD+ to occur efficiently, even in harsh chemical environments. Moreover, we show that regeneration of NAD+ by the photocatalyst nanoparticles can oxidize a model substrate, in conjunction with the enzyme glycerol dehydrogenase. To ensure the longevity of the enzyme, we immobilized it within a protective silica matrix; this yielded enzymatic silica nanoparticles with enhanced long-term performance and compatibility with the NAD+ -regeneration system.