Structural elucidation of a branch-on-branch β-glucan from Hericium erinaceus with A HPAEC-PAD-MS system.
Carbohydrate polymers
confidence
Key findings
Structural elucidation of a branch-on-branch beta-glucan from Hericium erinaceus using HPAEC-PAD-MS; no clinical or biological endpoints reported.
View source on PubMed (PMID 33142623) ↗
- Sample size
- Not reported
- Population
- Not applicable (analytical chemistry study of Hericium erinaceus glucan)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- nootropic
Full abstract
A water-soluble β-glucan from fruiting body of Hericium erinaceus was obtained after water extraction, purification and fractionation. Analyses of monosaccharide composition, molecular weight and linkage mode demonstrated that it is a β-glucan with 1→3 and 1→6 linkage modes and a molecular weight of 13.3 kDa. An endo-1,6-β-d-glucanase was used to digest the β-glucan and the digested products over time were analyzed with a HPAEC-PAD-MS platform. The linkage mode of each glycosidic bond in the digested oligosaccharides were confirmed with MS/MS. At the end of digestion, enzyme resistant oligosaccharides were observed as several 1→6 linked glucoses with one or two 1→3 linked glucose residues. All these domains are more like constructional pieces from a branch-on-branch glucan, in which multiple 1→6 linked glucan chains are hooked through one or two 1→3 linked glucose residues. Averagely, there is a 1→3 linkage per six 1→6 linked glucoses in this branch-on-branch β-glucan.