VqBGH40a isolated from Chinese wild Vitis quinquangularis degrades trans-piceid and enhances trans-resveratrol.
Plant science : an international journal of experimental plant biology
confidence
Key findings
VqBGH40a hydrolyzes trans-piceid to trans-resveratrol in vitro and in vivo, enhancing resveratrol content and participating in grapevine defense against powdery mildew.
View source on PubMed (PMID 34315603) ↗
- Sample size
- Not reported
- Population
- Chinese wild grapevine Vitis quinquangularis accession Danfeng-2 (plant study)
- Dosing
- 0.1 mM IPTG induction; transient overexpression in leaves
- Duration
- Not reported
- Route
- Prokaryotic expression system / transient overexpression in plant leaves
- Blinding
- not_reported
- Controls
- none
- Drug class
- polyphenol
Full abstract
Resveratrol (3,5,4'-trihydroxy-stilbene) is a phytoalexin that can prevent plants from pathogen attacks. Piceid is the glycosylation product of resveratrol and the main storage form of stilbenes in grapevines. Here, we reported the function of a β-glycoside hydrolase gene, VqBGH40a, from the Chinese wild grapevine Vitis quinquangularis accession Danfeng-2 in the regulation of plant resistance to powdery mildew (Uncinula necator). VqBGH40a belonging to β-glycoside hydrolase family 1 encoded 506 amino acids and was located on the cytomembrane. Its optimal induction condition was 28 or 30℃, for 4 h, with 0.1 mM IPTG in a prokaryotic expression system. Enzyme activity detection showed that purified VqBGH40a could hydrolyze trans-piceid to form trans-resveratrol in vitro. VqBGH40a was transiently overexpressed in Danfeng-2 leaves and then artificially inoculated with powdery mildew showed that VqBGH40a protein could hydrolyze trans-piceid in vivo. Additionally, a comparative family analysis between VqBGH40a and 38 VviBGHs was performed. Overall, these results demonstrate that VqBGH40a can hydrolyze trans-piceid, enhance trans-resveratrol content, and participate in the defense mechanism of grapevine against powdery mildew.