Senolyticsreview2024
Brief guide to senescence assays using cultured mammalian cells.
Molecules and cells
confidence
Key findings
Brief guide to senescence assays (cell cycle arrest, morphology, SA-β-gal, SASP); no clinical/biological endpoints reported.
View source on PubMed (PMID 39053732) ↗
- Sample size
- Not reported
- Population
- Cultured mammalian cells (in vitro assay guide)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- not_reported
- Drug class
- senolytic class
Full abstract
Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.