Rheological Characterization of Marine and Bovine Collagen Peptides/Acetic Acid Slurries Incorporating Hydroxyapatite Nanoparticles.
Polymers
confidence
Key findings
Fish-derived collagen peptide/HA slurries showed higher viscosity, modulus, and microstructural uniformity than bovine; suitable for 3D printing.
View source on PubMed (PMID 41374883) ↗
- Sample size
- Not reported
- Population
- In vitro collagen peptide/Hydroxyapatite nanocomposite slurries
- Dosing
- HA nanoparticles in acetic acid solutions of bovine or fish-derived collagen peptides
- Duration
- Over ten days of aging
- Route
- Not applicable (in vitro)
- Blinding
- not_reported
- Controls
- none
- Drug class
- peptide
Full abstract
The development of collagen-based composite materials for bone tissue engineering requires a comprehensive understanding of their rheological and structural behavior to ensure processability and functional stability. This study investigates the viscoelastic and morphological properties of nanocomposite slurries composed of hydroxyapatite (HA) nanoparticles dispersed in acetic acid solutions of bovine or fish-derived collagen peptides. Frequency and strain sweep tests revealed solid-like behavior and shear-thinning characteristics consistent with printable bioinks. Both formulations yield stresses between 0.7 and 1.5 kPa, values comparable to those reported for 3D-printable HA composites. Over ten days of aging, fish-based formulations retained higher viscosity and modulus, indicating improved temporal stability relative to bovine-based ones. Drop-casting tests confirmed the formation of homogeneous, highly opalescent films, with surface profilometry showing lower waviness for the fish-derived blend, suggesting enhanced microstructural uniformity. These results demonstrate that acetic acid-mediated collagen-HA interactions generate stable, high-fidelity slurries suitable for additive manufacturing applications. The superior rheological properties of fish collagen formulations highlight the influence of peptide source on network evolution, offering valuable insight for optimizing collagen-ceramic composites in regenerative and biomedical applications.