High pressure liquid chromatographic determination of vitamin D3 in instant nonfat dried milk.
Journal - Association of Official Analytical Chemists
confidence
Key findings
Analytical HPLC method for vitamin D3 determination in milk; 97.7% recovery at 10000 IU/kg, SD 3.9%. No clinical/biological endpoints.
View source on PubMed (PMID 6251024) ↗
- Sample size
- Not reported
- Population
- Commercially fortified instant nonfat dried milk (analytical sample)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- Not reported
- Blinding
- not_reported
- Controls
- none
- Drug class
- fat-soluble vitamin
Full abstract
Vitamin D3 was determined in commercially fortified instant nonfat dried milk by using normal phase high pressure liquid chromatography (HPLC). The sample was extracted with dichloromethane with sodium phosphate tribasic solution added. The sample was cleaned up by using a Sep-Pak silica cartridge and then a microparticulate column containing 10 micrometer Partisil-10 PAC packing material. The final analysis was performed by using a normal phase HPLC system with 10 micrometer LiChrosorb NH2 column. Recovery of vitamin D3 at levels as low as 10000 IU/kg was 97.7% with a standard deviation of 3.9%.