Conformational transition of hyaluronic acid: carboxylic group participation and thermal effect.
Biochimica et biophysica acta
confidence
Key findings
Analytical study of hyaluronic acid conformational transition in mixed solvent; no clinical or biological endpoints reported.
View source on PubMed (PMID 667126) ↗
- Sample size
- Not reported
- Population
- In vitro hyaluronic acid in mixed organic/water solvent
- Dosing
- Not applicable
- Duration
- Not reported
- Route
- Not applicable
- Blinding
- not_reported
- Controls
- none
- Drug class
- glycosaminoglycan
Full abstract
The chiroptical, viscosity and titration studies of hyaluronic acid in mixed organic/water solvent show a reversible conformational transition of the molecule depending upon pH, solvent composition, temperature, and molecular weight. Neither methylhyaluronate nor chondroitin undergoes conformational transition of this type. These results indicate that hydrogen bonding between the protonated carboxylic group and carbonyl oxygen of the acetamido group is directly involved in the conformational change. Results with chondroitin provide further support for the 4-fold helical structure that we have proposed for hyaluronic acid in mixed organic/water solvent. The thermal stability of the conformation has been studied under various pH values and solvent compositions.