[Isolation and properties of NAD-dependent formate dehydrogenase from the yeast Candida methylica].
Biokhimiia (Moscow, Russia)
confidence
Key findings
Biochemical characterization of NAD-dependent formate dehydrogenase from Candida methylica; no clinical or biological endpoints reported.
View source on PubMed (PMID 7260197) ↗
- Population
- Cell-free extract of methanol-consuming yeast Candida methylica (in vitro enzyme purification)
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
NAD-dependent formate dehydrogenase was purified from a cell-free extract of methanol-consuming yeast Candida methylica, using chromatography on DEAE-cellulose and gel-filtration on Sephadex G-200. The enzyme is electrophoretically homogeneous, consists of two identical subunits with molecular weight of 46,000 and is active within the pH range of 6-9; the Km values for NAD and formate are 1 . 10(-4) and 1,3 . 10(-2) M, respectively. Formate dehydrogenase is inhibited by p-chloromercurybenzoate, iodoacetate, dithionitrobenzoate and azide.