Glycine transport into plasma-membrane vesicles derived from rat brain synaptosomes.
The Biochemical journal
confidence
Key findings
Glycine transport into rat brain membrane vesicles is Na+/Cl--dependent, driven by ion gradients; no clinical/biological endpoints reported.
View source on PubMed (PMID 7326021) ↗
- Sample size
- Not reported
- Population
- Rat brain synaptosomes (in vitro membrane vesicles)
- Dosing
- Not reported
- Duration
- Not reported
- Route
- In vitro
- Blinding
- not_reported
- Controls
- none
- Drug class
- amino acid
Full abstract
1. Transport of glycine has been demonstrated in membrane vesicles isolated from rat brain, using artificially imposed ion gradients as the sole energy source. 2. The uptake of glycine is strictly dependent on the presence of Na+ and Cl- in the medium, and the process can be driven either by an Na+ gradient (out greater than in) or by a C1- gradient (out greater than in) when the other essential ion is present. 3. The uptake of glycine is stimulated by a membrane potential (interior negative), as demonstrated by the effects of the ionophores valinomycin and carbonyl cyanide m-chlorophenylhydrazone and anions of different permeabilities. 4. The kinetic analysis shows that glycine is accumulated by two systems with different affinities. 5. The presence of ouabain, an inhibitor (Na+ + K+)-activated ATPase, does not affect glycine transport. 6. The existence of a high-affinity, Na+-dependent glycine-uptake system in membrane vesicles derived from rat brain suggests that this amino acid may have a transmitter role in some areas of the rat brain.