NAD+animalAnimal model1996
Rat liver mitochondria can synthesize nicotinamide adenine dinucleotide from nicotinamide mononucleotide and ATP via a putative matrix nicotinamide mononucleotide adenylyltransferase.
Biochemistry and molecular biology international
confidence
Key findings
Mitochondrial matrix contains a specific NMN adenylyltransferase (E.C. 2.7.7.1.) that synthesizes NAD from NMN and ATP; biochemical enzyme study.
View source on PubMed (PMID 8850525) ↗
- Population
- Rat liver mitochondria (in vitro)
- Dosing
- NMN added to mitochondria
- Route
- in vitro
- Blinding
- not_reported
- Controls
- none
- Drug class
- coenzyme
Full abstract
In order to gain some insight into the mechanism by which nicotinamide nucleotides localize in mitochondria, NMN was added to rat liver mitochondria, with NAD synthesis tested both enzymatically and by means of HPLC. Evidence is given that the mitochondrial matrix contains a specific NMN adenylyltransferase (E.C. 2.7.7.1.), inhibited by PPi, AMP and ADP-ribose. Some features of this enzyme, including the substrate, pH and temperature dependence were also investigated.